Hotspot

psbI-trnS-GCU

LSC · 8220-8334 · 115 bp

Back to Ajuga

Pi 0.0349
MarkerSeek score 69.10
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
115
variable_sites
14
pi
0.0349
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 ATCTAATGATCCGGGGCGTA TGGACTAAAGCGTCGGATTG 50.0 50.0 58.1 58.0 223-244 bp 1.0 86.4
psbI-trnS-GCU_p2 TATCTAATGATCCGGGGCGT TGGACTAAAGCGTCGGATTG 50.0 50.0 58.1 58.0 224-245 bp 1.0 86.4
psbI-trnS-GCU_p3 ATCTAATGATCCGGGGCGTA GTGGACTAAAGCGTCGGATT 50.0 50.0 58.1 58.0 224-245 bp 1.0 86.4
psbI-trnS-GCU_p4 TATCTAATGATCCGGGGCGT GTGGACTAAAGCGTCGGATT 50.0 50.0 58.1 58.0 225-246 bp 1.0 86.3
psbI-trnS-GCU_p5 ATCTAATGATCCGGGGCGTA ATCTTGGGAGAGATGGCTGA 50.0 50.0 58.1 57.8 244-265 bp 1.0 84.4