Hotspot

rps16-trnQ-UUG

LSC · 6314-7134 · 821 bp

Back to Ajuga

Pi 0.0142
MarkerSeek score 68.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
821
variable_sites
56
pi
0.0142
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGGATCGTGTCCTTCAAG TCGGAGGTTCGAATCCTTTC 50.0 50.0 57.9 57.3 521-1310 bp 1.0 83.0
rps16-trnQ-UUG_p2 AACGGATCGTGTCCTTCAAG TAAGGCAACGGGTTTTGGTC 50.0 50.0 57.9 58.7 548-1337 bp 1.0 82.7
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TCGGAGGTTCGAATCCTTTC 45.0 50.0 58.1 57.3 504-1293 bp 1.0 82.6
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 531-1320 bp 1.0 82.3
rps16-trnQ-UUG_p5 AGCATCCCCATTCCTAGACA TCGGAGGTTCGAATCCTTTC 50.0 50.0 57.8 57.3 570-1359 bp 1.0 82.2