Hotspot

trnK-UUU-rps16

LSC · 4264-5156 · 893 bp

Back to Ajuga

Pi 0.0107
MarkerSeek score 70.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
893
variable_sites
46
pi
0.0107
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT CCACAGGAACCGTTCAGAAT 50.0 50.0 58.2 57.8 977-1014 bp 1.0 84.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT CCACAGGAACCGTTCAGAAT 50.0 50.0 58.2 57.8 976-1013 bp 1.0 84.9
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC CCACAGGAACCGTTCAGAAT 50.0 50.0 58.3 57.8 991-1028 bp 1.0 84.1
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA CCACAGGAACCGTTCAGAAT 50.0 50.0 58.3 57.8 986-1023 bp 1.0 84.0
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC CCACAGGAACCGTTCAGAAT 50.0 50.0 57.5 57.8 963-1000 bp 1.0 82.6