Hotspot

ndhF

SSC · 107810-110032 · 2223 bp

Pi 0.00195

MarkerSeek score 44.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 13
pi: 0.00195
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTAGCACTGGGTAGACCTCA	AGCTAAGATCCGGTGAATCG	55.0	50.0	57.9	57.2	2629-2669 bp	1.0	73.4
ndhF_p2	TCGTTGTGGTCGGACTCTAT	AGCTAAGATCCGGTGAATCG	50.0	50.0	58.2	57.2	2808-2848 bp	1.0	73.1
ndhF_p3	ATCAATTCGGTCGTTGTGGT	AGCTAAGATCCGGTGAATCG	45.0	50.0	57.8	57.2	2818-2858 bp	1.0	73.0
ndhF_p4	CTAGCACTGGGTAGACCTCA	CGAAAGAGCTAAGATCCGGT	55.0	50.0	57.9	57.1	2635-2675 bp	1.0	72.8
ndhF_p5	TCGTTGTGGTCGGACTCTAT	CGAAAGAGCTAAGATCCGGT	50.0	50.0	58.2	57.1	2814-2854 bp	1.0	72.6