Hotspot

rpl32-trnL-UAG

SSC · 111187-112067 · 881 bp

Back to Ajania

Pi 0.00764
MarkerSeek score 61.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
881
variable_sites
19
pi
0.00764
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCGGCATTAAAAGCTTTGT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 989-1001 bp 1.0 81.9
rpl32-trnL-UAG_p2 GGCGGCATTAAAAGCTTTGT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 990-1002 bp 1.0 81.9
rpl32-trnL-UAG_p3 AAAGGATATTGGGCGGCATT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 1000-1012 bp 1.0 81.8
rpl32-trnL-UAG_p4 AAAGGATATTGGGCGGCATT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 1001-1013 bp 1.0 81.8
rpl32-trnL-UAG_p5 GGCGGCATTAAAAGCTTTGT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 999-1011 bp 1.0 80.9