Hotspot

ndhC-trnV-UAC

LSC · 49901-51053 · 1153 bp

Back to Ajania

Pi 0.0029
MarkerSeek score 54.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1153
variable_sites
10
pi
0.0029
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTTTGCTACTCGGAGCTA GAAGGTCTACGGTTCGATCC 50.0 55.0 57.9 57.5 1293-1319 bp 1.0 76.3
ndhC-trnV-UAC_p2 ACTCGGAGCTAAAAATCCCG GAAGGTCTACGGTTCGATCC 50.0 55.0 57.7 57.5 1284-1310 bp 1.0 74.9
ndhC-trnV-UAC_p3 TTCTCCGGTCCTTTGCTACT GAAGGTCTACGGTTCGATCC 50.0 55.0 58.4 57.5 1301-1327 bp 1.0 74.5
ndhC-trnV-UAC_p4 TCCTTTGCTACTCGGAGCTA GTTCGATCCCGTATAGCCCT 50.0 55.0 57.9 58.7 1282-1308 bp 1.0 74.2
ndhC-trnV-UAC_p5 TTTCTCCGGTCCTTTGCTAC GAAGGTCTACGGTTCGATCC 50.0 55.0 57.5 57.5 1302-1328 bp 1.0 73.6