Hotspot

trnR-UCU-trnG-UCC

LSC · 29700-29909 · 210 bp

Back to Ajania

Pi 0.00962
MarkerSeek score 58.20
Primer available yes
Label trnR-UCU-trnG-UCC

Feature Metadata

label_name
trnR-UCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
210
variable_sites
4
pi
0.00962
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnG-UCC_p1 AGAAGACCTCTGTCCTATCCA AGCCTTCCAAGCTAACGATG 47.6 50.0 56.9 58.0 278-292 bp 1.0 68.5
trnR-UCU-trnG-UCC_p2 AGAAGACCTCTGTCCTATCCA CCTAGCCTTCCAAGCTAACG 47.6 55.0 56.9 58.1 281-295 bp 1.0 68.2
trnR-UCU-trnG-UCC_p3 AGGTTTAGAAGACCTCTGTCCT AGCCTTCCAAGCTAACGATG 45.5 50.0 57.9 58.0 284-298 bp 1.0 67.8
trnR-UCU-trnG-UCC_p4 AGGTTTAGAAGACCTCTGTCCT CCTAGCCTTCCAAGCTAACG 45.5 55.0 57.9 58.1 287-301 bp 1.0 67.5
trnR-UCU-trnG-UCC_p5 AGAAGACCTCTGTCCTATCCA CCCTAGCCTTCCAAGCTAAC 47.6 55.0 56.9 57.7 282-296 bp 1.0 66.0