Hotspot

petN-psbM

LSC · 10016-10544 · 529 bp

Back to Ajania

Pi 0.00529
MarkerSeek score 55.80
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
529
variable_sites
6
pi
0.00529
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTT 50.0 40.0 58.2 55.6 686-691 bp 1.0 60.7
petN-psbM_p2 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTTT 50.0 38.1 58.2 56.4 686-691 bp 1.0 59.0
petN-psbM_p3 ATAGTAAGTCTCGCTTGGGC TGCTACTGCACTGTTCATTT 50.0 40.0 57.1 55.6 687-692 bp 1.0 54.8
petN-psbM_p4 CTCGCTTGGGCTGCTTTAAT TGCTACTGCACTGTTCATTT 50.0 40.0 58.9 55.6 678-683 bp 1.0 54.7
petN-psbM_p5 TGGGCTGCTTTAATGGTAGTC TGCTACTGCACTGTTCATTT 47.6 40.0 58.0 55.6 672-677 bp 1.0 53.9