Hotspot

rps16-trnQ-UUG

LSC · 6326-7213 · 888 bp

Back to Ajania

Pi 0.00323
MarkerSeek score 61.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
888
variable_sites
8
pi
0.00323
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 961 bp 1.0 78.9
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 972 bp 1.0 78.4
rps16-trnQ-UUG_p3 CACACCGGATCATGTCCTTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.3 58.1 981 bp 1.0 77.3
rps16-trnQ-UUG_p4 CACCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 979 bp 1.0 76.8
rps16-trnQ-UUG_p5 CCAAGAGCACCTTCATTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1032 bp 1.0 76.0