Hotspot

ccsA-trnL-UAG

Genome · 122818-122932 · 115 bp

Back to Airampoa

Pi 0.0348

MarkerSeek score 66.40

Primer available yes

Label ccsA-trnL-UAG

Feature Metadata

label_name: ccsA-trnL-UAG
feature_type: igs
region: Genome
length_bp: 115
variable_sites: 4
pi: 0.0348
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-trnL-UAG_p1	TGGAAACCATTGAAAAGGAA	CTCTTAGGAAGCAGTGCGAG	35.0	55.0	53.1	58.1	223 bp	1.0	41.9
ccsA-trnL-UAG_p2	TGGAAACCATTGAAAAGGAA	CTTAGGAAGCAGTGCGAGAG	35.0	55.0	53.1	58.1	221 bp	1.0	41.9
ccsA-trnL-UAG_p3	TGGAAACCATTGAAAAGGAA	TAGACACGCTGCTCTTAGGA	35.0	50.0	53.1	57.9	234 bp	1.0	41.4
ccsA-trnL-UAG_p4	TGGAAACCATTGAAAAGGAAA	CTCTTAGGAAGCAGTGCGAG	33.3	55.0	54.1	58.1	223 bp	1.0	41.4
ccsA-trnL-UAG_p5	TGGAAACCATTGAAAAGGAAA	CTTAGGAAGCAGTGCGAGAG	33.3	55.0	54.1	58.1	221 bp	1.0	41.4