Hotspot

petA-psbJ

Genome · 80384-81477 · 1094 bp

Back to Airampoa

Pi 0.00478

MarkerSeek score 57.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 1094
variable_sites: 5
pi: 0.00478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGAGAAGGTTCAATTGTCCG	ACTGGCATTCTTGTGATCGT	50.0	45.0	56.8	57.5	1157-1203 bp	1.0	70.5
petA-psbJ_p2	CGAGAAGGTTCAATTGTCCG	CGTATTCTGGATTGGGCTCA	50.0	50.0	56.8	57.4	1111-1157 bp	1.0	69.5
petA-psbJ_p3	CGAGAAGGTTCAATTGTCCGA	ACTGGCATTCTTGTGATCGT	47.6	45.0	58.3	57.5	1157-1203 bp	1.0	69.3
petA-psbJ_p4	TCGAGAAGGTTCAATTGTCCG	ACTGGCATTCTTGTGATCGT	47.6	45.0	58.3	57.5	1158-1204 bp	1.0	69.3
petA-psbJ_p5	CGAGAAGGTTCAATTGTCCG	ATTCTGGATTGGGCTCATCC	50.0	50.0	56.8	57.3	1108-1154 bp	1.0	69.1