Hotspot

ycf4-cemA

Genome · 77484-78465 · 982 bp

Back to Airampoa

Pi 0.00306

MarkerSeek score 60.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: Genome
length_bp: 982
variable_sites: 3
pi: 0.00306
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CATTCCCTTGACCCGTACTG	AGAGAGATCCACCAGGGTAA	55.0	50.0	58.0	56.7	1152-1153 bp	1.0	70.1
ycf4-cemA_p2	CATTCCCTTGACCCGTACTG	TGATAGAGAGATCCACCAGGG	55.0	52.4	58.0	57.7	1156-1157 bp	1.0	70.1
ycf4-cemA_p3	CATTCCCTTGACCCGTACTG	GATAGAGAGATCCACCAGGGT	55.0	52.4	58.0	57.7	1155-1156 bp	1.0	70.1
ycf4-cemA_p4	ATTCCCTTGACCCGTACTGA	AGAGAGATCCACCAGGGTAA	50.0	50.0	58.1	56.7	1151-1152 bp	1.0	69.8
ycf4-cemA_p5	TTCCCTTGACCCGTACTGAT	AGAGAGATCCACCAGGGTAA	50.0	50.0	58.1	56.7	1150-1151 bp	1.0	69.8