Hotspot

accD-psaI

Genome · 75863-76519 · 657 bp

Back to Airampoa

Pi 0.00457

MarkerSeek score 61.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: Genome
length_bp: 657
variable_sites: 3
pi: 0.00457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GGTGCACAAACAACCGAAAA	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	832-834 bp	1.0	80.1
accD-psaI_p2	GGTGCACAAACAACCGAAAA	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	838-840 bp	1.0	79.5
accD-psaI_p3	TCGATCCAGTCTTACCACGT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	792-794 bp	1.0	78.8
accD-psaI_p4	TGAGTTACTTGAGCTGCACG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	749-751 bp	1.0	78.5
accD-psaI_p5	TCGATCCAGTCTTACCACGT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	798-800 bp	1.0	78.2