Hotspot

trnF-GAA-ndhJ

Genome · 63127-64100 · 974 bp

Back to Airampoa

Pi 0.0031

MarkerSeek score 61.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: Genome
length_bp: 974
variable_sites: 3
pi: 0.0031
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	1111-1116 bp	1.0	80.0
trnF-GAA-ndhJ_p2	GAGTAGATGTTGCGCCAGAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.0	58.1	1143-1148 bp	1.0	79.9
trnF-GAA-ndhJ_p3	AGTAGATGTTGCGCCAGAAG	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.0	58.1	1142-1147 bp	1.0	79.9
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	1075-1080 bp	1.0	79.6
trnF-GAA-ndhJ_p5	ATGAGTAGATGTTGCGCCAG	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	1145-1150 bp	1.0	79.6