Hotspot

trnS-GGA-rps4

Genome · 59669-60023 · 355 bp

Back to Airampoa

Pi 0.0113

MarkerSeek score 53.10

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: Genome
length_bp: 355
variable_sites: 4
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	ACCCTTCACCCATTCCCATA	50.0	50.0	58.1	58.0	509 bp	1.0	79.8
trnS-GGA-rps4_p2	CCGAGTGGTTTAAGGCGTAG	ACCCTTCACCCATTCCCATA	55.0	50.0	58.4	58.0	546 bp	1.0	77.6
trnS-GGA-rps4_p3	GGCTTTTGTTTACCGAGGGT	ACCCTTCACCCATTCCCATA	50.0	50.0	58.4	58.0	508 bp	1.0	77.5
trnS-GGA-rps4_p4	AGGCTTTTGTTTACCGAGGG	TTTGACCCTTCACCCATTCC	50.0	50.0	58.1	57.7	513 bp	1.0	77.4
trnS-GGA-rps4_p5	GAGATGGCCGAGTGGTTTAA	ACCCTTCACCCATTCCCATA	50.0	50.0	57.6	58.0	553 bp	1.0	77.4