Hotspot

psbB-clpP

Genome · 20952-21961 · 1010 bp

Back to Airampoa

Pi 0.00496

MarkerSeek score 47.40

Primer available yes

Label psbB-clpP

Feature Metadata

label_name: psbB-clpP
feature_type: igs
region: Genome
length_bp: 1010
variable_sites: 5
pi: 0.00496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-clpP_p1	AATGCGCCAAAACCAAAACA	TTATCTCTAGAGCGGGTGGG	40.0	55.0	58.0	58.0	1721-1725 bp	1.0	80.1
psbB-clpP_p2	ATGCGCCAAAACCAAAACAA	TTATCTCTAGAGCGGGTGGG	40.0	55.0	58.0	58.0	1720-1724 bp	1.0	80.1
psbB-clpP_p3	ATCCCAATACACCCAATGCC	TTATCTCTAGAGCGGGTGGG	50.0	55.0	57.9	58.0	1611-1615 bp	1.0	79.6
psbB-clpP_p4	AATGCGCCAAAACCAAAACA	ATCTGCCCATAGAACCGAGA	40.0	50.0	58.0	57.9	1545-1546 bp	1.0	79.4
psbB-clpP_p5	ATGCGCCAAAACCAAAACAA	ATCTGCCCATAGAACCGAGA	40.0	50.0	58.0	57.9	1544-1545 bp	1.0	79.4