Hotspot

ndhD

SSC · 105928-107430 · 1503 bp

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Pi 0.002
MarkerSeek score 59.90
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1503
variable_sites
3
pi
0.002
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 TCTGCATTAATAGAGCGGACT CGGGCTTTTCTGGTCAAAAC 42.9 50.0 56.6 57.9 1612 bp 1.0 60.0
ndhD_p2 TCTGCATTAATAGAGCGGACT GGGCTTTTCTGGTCAAAACG 42.9 50.0 56.6 57.9 1611 bp 1.0 60.0
ndhD_p3 TCTGCATTAATAGAGCGGACT ACGGGCTTTTCTGGTCAAAA 42.9 45.0 56.6 58.2 1613 bp 1.0 59.2
ndhD_p4 TCTGCATTAATAGAGCGGACTT CGGGCTTTTCTGGTCAAAAC 40.9 50.0 57.3 57.9 1612 bp 1.0 57.7
ndhD_p5 TCTGCATTAATAGAGCGGACTT GGGCTTTTCTGGTCAAAACG 40.9 50.0 57.3 57.9 1611 bp 1.0 57.7