Hotspot

rpl32-trnL-UAG

SSC · 103847-104621 · 775 bp

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Pi 0.0103
MarkerSeek score 64.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
775
variable_sites
8
pi
0.0103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTCCAGCGGCAATGAGTATC TTCGAACCGAGATGCTTCAG 50.0 50.0 58.0 58.0 902-913 bp 1.0 80.3
rpl32-trnL-UAG_p2 CATTTTCCAGCGGCAATGAG TTCGAACCGAGATGCTTCAG 50.0 50.0 58.1 58.0 906-917 bp 1.0 80.0
rpl32-trnL-UAG_p3 TTCCAGCGGCAATGAGTATC GGATTCGAACCGAGATGCTT 50.0 50.0 58.0 58.1 905-916 bp 1.0 79.9
rpl32-trnL-UAG_p4 CATTTTCCAGCGGCAATGAG GGATTCGAACCGAGATGCTT 50.0 50.0 58.1 58.1 909-920 bp 1.0 79.6
rpl32-trnL-UAG_p5 TTCCAGCGGCAATGAGTATC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 875-886 bp 1.0 79.4