Hotspot

ndhF-rpl32

SSC · 102740-103666 · 927 bp

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Pi 0.00216
MarkerSeek score 60.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
927
variable_sites
2
pi
0.00216
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TGCTAAAGAATAAGCGTGTACT 55.0 36.4 58.1 55.6 1082-1102 bp 1.0 43.9
ndhF-rpl32_p2 TAAGGCCAAACCCGATTGAC TGCTAAAGAATAAGCGTGTACT 50.0 36.4 58.2 55.6 1111-1131 bp 1.0 43.4
ndhF-rpl32_p3 GGCCAAACCCGATTGACATA TGCTAAAGAATAAGCGTGTACT 50.0 36.4 58.2 55.6 1108-1128 bp 1.0 42.9
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTGCTAAAGAATAAGCGTGTACT 55.0 34.8 58.1 56.3 1083-1103 bp 1.0 41.7
ndhF-rpl32_p5 TAAGGCCAAACCCGATTGAC TTGCTAAAGAATAAGCGTGTACT 50.0 34.8 58.2 56.3 1112-1132 bp 1.0 41.2