Hotspot

psaJ-rpl33

LSC · 63558-64011 · 454 bp

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Pi 0.011
MarkerSeek score 62.00
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
454
variable_sites
5
pi
0.011
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 CCAGATGCTTTGTCATTCCC GAAATCCCCGTCGATTCCTC 50.0 55.0 56.8 58.1 576 bp 1.0 69.7
psaJ-rpl33_p2 CCAGATGCTTTGTCATTCCCT GAAATCCCCGTCGATTCCTC 47.6 55.0 58.3 58.1 576 bp 1.0 69.5
psaJ-rpl33_p3 CCAGATGCTTTGTCATTCCC GGCACCTTTTCGAACACAAC 50.0 50.0 56.8 58.2 553 bp 1.0 69.5
psaJ-rpl33_p4 TCCAGATGCTTTGTCATTCCC GAAATCCCCGTCGATTCCTC 47.6 55.0 58.3 58.1 577 bp 1.0 69.4
psaJ-rpl33_p5 CCAGATGCTTTGTCATTCCCT GGCACCTTTTCGAACACAAC 47.6 50.0 58.3 58.2 553 bp 1.0 69.3