Hotspot

petN-trnC-GCA

LSC · 17155-18047 · 893 bp

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Pi 0.00448
MarkerSeek score 61.30
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
893
variable_sites
4
pi
0.00448
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 AGTCCACTTCTCCCCCATAC AGTTCAAATCTGGGTGCCG 55.0 52.6 58.1 58.1 1001 bp 1.0 70.9
petN-trnC-GCA_p2 GTCCACTTCTCCCCCATACT AGTTCAAATCTGGGTGCCG 55.0 52.6 58.1 58.1 1000 bp 1.0 70.9
petN-trnC-GCA_p3 AGCCCAAGCGAGACTTACTA AGTTCAAATCTGGGTGCCG 50.0 52.6 58.2 58.1 942 bp 1.0 70.7
petN-trnC-GCA_p4 AGTCCACTTCTCCCCCATAC CCAGTTCAAATCTGGGTGCC 55.0 55.0 58.1 59.1 1003 bp 1.0 70.5
petN-trnC-GCA_p5 GTCCACTTCTCCCCCATACT CCAGTTCAAATCTGGGTGCC 55.0 55.0 58.1 59.1 1002 bp 1.0 70.5