Hotspot

ycf1

SSC · 124246-129750 · 5505 bp

Back to Aiphanes

Pi 0.00747

MarkerSeek score 46.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5505
variable_sites: 41
pi: 0.00747
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTGGCACAAATTCTGGGAG	TGCTACACATAAAAATCTGGA	50.0	33.3	58.1	52.8	213 bp	1.0	42.3
ycf1_p2	AGAGTGGCACAAATTCTGGG	TGCTACACATAAAAATCTGGA	50.0	33.3	58.1	52.8	215 bp	1.0	42.2
ycf1_p3	GAGTGGCACAAATTCTGGGA	TGCTACACATAAAAATCTGGA	50.0	33.3	58.1	52.8	214 bp	1.0	42.2
ycf1_p4	AGTGGCACAAATTCTGGGAG	TTGCTACACATAAAAATCTGGA	50.0	31.8	58.1	53.7	214 bp	1.0	42.2
ycf1_p5	ATGCATCAAAGAGTGGCACA	TGCTACACATAAAAATCTGGA	45.0	33.3	58.1	52.8	224 bp	1.0	42.1