Hotspot

rps8

LSC · 80087-80485 · 399 bp

Back to Aiphanes

Pi 0.01

MarkerSeek score 52.20

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 399
variable_sites: 4
pi: 0.01
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	AGGATACCAGAGGAGGAGGA	TCAAAATTAGGAGGCACAAA	55.0	35.0	58.1	53.0	454 bp	1.0	40.7
rps8_p2	AGGATACCAGAGGAGGAGGA	TCAAAATTAGGAGGCACAAAA	55.0	33.3	58.1	53.9	454 bp	1.0	40.7
rps8_p3	AGGATACCAGAGGAGGAGGA	TCAAAATTAGGAGGCACAAAAA	55.0	31.8	58.1	54.7	454 bp	1.0	40.7
rps8_p4	AGGATACCAGAGGAGGAGGA	ATCAAAATTAGGAGGCACAA	55.0	35.0	58.1	52.7	455 bp	1.0	40.7
rps8_p5	AGGATACCAGAGGAGGAGGA	ATCAAAATTAGGAGGCACAAA	55.0	33.3	58.1	53.6	455 bp	1.0	40.7