Hotspot

rps15-ycf1

SSC · 123863-124245 · 383 bp

Back to Aiphanes

Pi 0.0157

MarkerSeek score 48.80

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 383
variable_sites: 6
pi: 0.0157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GGCTCAGCTCCAGCATAATT	CGGCAATATGCTTGTGAAGG	50.0	50.0	58.0	57.5	1459-1460 bp	1.0	76.7
rps15-ycf1_p2	AACGAGTTTTTGGTTCCCGA	CGGCAATATGCTTGTGAAGG	45.0	50.0	58.0	57.5	1029-1030 bp	1.0	76.6
rps15-ycf1_p3	ACGAGTTTTTGGTTCCCGAA	CGGCAATATGCTTGTGAAGG	45.0	50.0	58.0	57.5	1028-1029 bp	1.0	76.6
rps15-ycf1_p4	AACACCATGCATTGATGGGT	CGGCAATATGCTTGTGAAGG	45.0	50.0	58.1	57.5	1206-1207 bp	1.0	76.6
rps15-ycf1_p5	GAAGCTATACGGCTCAGCTC	CGGCAATATGCTTGTGAAGG	55.0	50.0	57.9	57.5	1469-1470 bp	1.0	76.4