Hotspot

rpl22-rps19

IRb · 84001-84132 · 132 bp

Back to Aiphanes

Pi 0.0379

MarkerSeek score 72.20

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 132
variable_sites: 5
pi: 0.0379
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTCCGCGAATCTGATCAAT	TCTGACTTTCGCGAGACATG	50.0	50.0	58.5	58.0	297-309 bp	1.0	77.1
rpl22-rps19_p2	CGTCCGCGAATCTGATCAAT	CCTACTCTGACTTTCGCGAG	50.0	55.0	58.5	57.8	302-314 bp	1.0	75.8
rpl22-rps19_p3	CGTCCGCGAATCTGATCAAT	CTCTGACTTTCGCGAGACAT	50.0	50.0	58.5	57.7	298-310 bp	1.0	75.1
rpl22-rps19_p4	CGTCCGCGAATCTGATCAAT	CAAATTGGGAGAATTCGCGC	50.0	50.0	58.5	58.5	321-333 bp	1.0	73.5
rpl22-rps19_p5	CGTCCGCGAATCTGATCAAT	TTCGCGAGACATGCAAGAAA	50.0	45.0	58.5	58.5	290-302 bp	1.0	73.3