Hotspot

rpoA-rps11

LSC · 78875-78953 · 79 bp

Back to Aiphanes

Pi 0.0253

MarkerSeek score 67.10

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 79
variable_sites: 2
pi: 0.0253
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	CCGTAGTGTTCGAGTGGATC	ATGGGTGTCGACCTCCTAAA	55.0	50.0	57.8	58.1	156 bp	1.0	79.1
rpoA-rps11_p2	CGTAGTGTTCGAGTGGATCC	AATGGGTGTCGACCTCCTAA	55.0	50.0	57.8	58.1	156 bp	1.0	79.1
rpoA-rps11_p3	CGTAGTGTTCGAGTGGATCC	ATGGGTGTCGACCTCCTAAA	55.0	50.0	57.8	58.1	155 bp	1.0	79.1
rpoA-rps11_p4	GGGACAGAATGAAACGACCA	AATGGGTGTCGACCTCCTAA	50.0	50.0	57.8	58.1	221 bp	1.0	78.9
rpoA-rps11_p5	GGGACAGAATGAAACGACCA	ATGGGTGTCGACCTCCTAAA	50.0	50.0	57.8	58.1	220 bp	1.0	78.9