Hotspot

ndhJ-ndhK

LSC · 48677-48766 · 90 bp

Back to Aiphanes

Pi 0.0222

MarkerSeek score 62.50

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 90
variable_sites: 2
pi: 0.0222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	TGGAGTACCAATCCTCGGTT	GTGAATCAGGCAGGGTTCTT	50.0	50.0	58.1	57.8	241 bp	1.0	78.6
ndhJ-ndhK_p2	TGGAGTACCAATCCTCGGTT	TCAGGCAGGGTTCTTTTGTG	50.0	50.0	58.1	58.3	236 bp	1.0	77.8
ndhJ-ndhK_p3	GCAATCGAAGCCCAAAGATC	GTGAATCAGGCAGGGTTCTT	50.0	50.0	57.5	57.8	195 bp	1.0	75.3
ndhJ-ndhK_p4	TGGAGTACCAATCCTCGGTT	AGTGAATCAGGCAGGGTTCT	50.0	50.0	58.1	58.6	242 bp	1.0	75.2
ndhJ-ndhK_p5	TGGAGTACCAATCCTCGGTT	ATCAGGCAGGGTTCTTTTGT	50.0	45.0	58.1	57.3	237 bp	1.0	74.8