Hotspot

trnK-UUU-rps16

LSC · 4129-4856 · 728 bp

Back to Aiphanes

Pi 0.00966

MarkerSeek score 64.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 728
variable_sites: 7
pi: 0.00966
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCTGGACGAATCTGTTGC	GCTCAACCTACGGGAACTG	50.0	57.9	57.9	57.9	897-899 bp	1.0	71.1
trnK-UUU-rps16_p2	GTCTGGACGAATCTGTTGCT	GCTCAACCTACGGGAACTG	50.0	57.9	57.9	57.9	896-898 bp	1.0	71.1
trnK-UUU-rps16_p3	AGTCTGGACGAATCTGTTGC	GTGCTCAACCTACGGGAAC	50.0	57.9	57.9	58.2	899-901 bp	1.0	70.9
trnK-UUU-rps16_p4	GTCTGGACGAATCTGTTGCT	GTGCTCAACCTACGGGAAC	50.0	57.9	57.9	58.2	898-900 bp	1.0	70.9
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	GCTCAACCTACGGGAACTG	50.0	57.9	58.2	57.9	840-842 bp	1.0	70.5