Hotspot

rpoC2

LSC · 17522-21679 · 4158 bp

Pi 0.000481

MarkerSeek score 54.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4158
variable_sites: 2
pi: 0.000481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	GGAACGGGGCATTAGTTCTT	TTTCTGACCTCTCCGTGACT	50.0	50.0	57.9	58.0	248 bp	1.0	79.3
rpoC2_p2	GGAACGGGGCATTAGTTCTT	TTCGCTCCACAAGGATCAAG	50.0	50.0	57.9	57.9	304 bp	1.0	78.5
rpoC2_p3	GGAACGGGGCATTAGTTCTT	CGCTCCACAAGGATCAAGAT	50.0	50.0	57.9	57.7	302 bp	1.0	76.7
rpoC2_p4	GGAACGGGGCATTAGTTCTT	CCTTTTTCGCTCCACAAGGA	50.0	50.0	57.9	58.4	309 bp	1.0	76.1
rpoC2_p5	GGAACGGGGCATTAGTTCTT	TAGTGCCTTTTTCGCTCCAC	50.0	50.0	57.9	58.5	314 bp	1.0	75.4