Hotspot

ndhK

LSC · 51327-52184 · 858 bp

Back to Aiouea

Pi 0.00117
MarkerSeek score 59.40
Primer available yes
Label ndhK

Feature Metadata

label_name
ndhK
feature_type
gene
region
LSC
length_bp
858
variable_sites
1
pi
0.00117
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhK_p1 ATGAACGACCCTGCATCTTC GGGGGATGCTTGGTTACAAT 50.0 50.0 58.0 57.8 1063 bp 1.0 78.6
ndhK_p2 GATGAACGACCCTGCATCTT GGGGGATGCTTGGTTACAAT 50.0 50.0 58.0 57.8 1064 bp 1.0 78.6
ndhK_p3 GAACGACCCTGCATCTTCTT GGGGGATGCTTGGTTACAAT 50.0 50.0 57.9 57.8 1061 bp 1.0 78.0
ndhK_p4 ATGAACGACCCTGCATCTTC GATGGGGGATGCTTGGTTAC 50.0 55.0 58.0 58.3 1066 bp 1.0 77.4
ndhK_p5 GATGAACGACCCTGCATCTT GATGGGGGATGCTTGGTTAC 50.0 55.0 58.0 58.3 1067 bp 1.0 77.4