Hotspot

ccsA

SSC · 118945-119895 · 951 bp

Pi 0

MarkerSeek score 27.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 951
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CGCGAGTGACCCAAATTCTA	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.0	58.0	1618 bp	1.0	80.1
ccsA_p2	TTATTTCGATGAAGCCGCCA	TTCTCGATGGCTTCTCTTGC	45.0	50.0	58.0	58.0	1849 bp	1.0	79.9
ccsA_p3	CGCGAGTGACCCAAATTCTA	CTCGATGGCTTCTCTTGCAT	50.0	50.0	58.0	58.0	1616 bp	1.0	79.9
ccsA_p4	CGCGAGTGACCCAAATTCTA	TTGCATTGCCAGGAATGAGT	50.0	45.0	58.0	58.1	1602 bp	1.0	79.7
ccsA_p5	TTATTTCGATGAAGCCGCCA	CTCGATGGCTTCTCTTGCAT	45.0	50.0	58.0	58.0	1847 bp	1.0	79.7