Hotspot

atpF

LSC · 13036-14317 · 1282 bp

Pi 0.000781

MarkerSeek score 59.30

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1282
variable_sites: 1
pi: 0.000781
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	GGGGGCGAAGTGATACAAAA	45.0	50.0	57.4	58.2	1444 bp	1.0	73.6
atpF_p2	ACTAATTTCGTCGGCTCGAA	CAATCCAAAACAAAGGGGGC	45.0	50.0	57.4	57.8	1458 bp	1.0	73.5
atpF_p3	AATTTCGTCGGCTCGAATGG	GGGGGCGAAGTGATACAAAA	50.0	50.0	59.0	58.2	1441 bp	1.0	70.8
atpF_p4	AATTTCGTCGGCTCGAATGG	CAATCCAAAACAAAGGGGGC	50.0	50.0	59.0	57.8	1455 bp	1.0	70.7
atpF_p5	ACTAATTTCGTCGGCTCGAA	CAAAGGGGGCGAAGTGATAC	45.0	55.0	57.4	58.6	1448 bp	1.0	70.0