Hotspot

ccsA-ndhD

SSC · 119896-120117 · 222 bp

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Pi 0.0225
MarkerSeek score 65.50
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
222
variable_sites
5
pi
0.0225
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GTGGATTCGGCAATTGTGG TCCTGATTTCGTTCTCTCGC 52.6 50.0 57.3 57.7 412 bp 1.0 65.0
ccsA-ndhD_p2 GTGGATTCGGCAATTGTGG CCTGATTTCGTTCTCTCGCT 52.6 50.0 57.3 57.7 411 bp 1.0 64.9
ccsA-ndhD_p3 TGGATTCGGCAATTGTGGC TCCTGATTTCGTTCTCTCGC 52.6 50.0 59.1 57.7 411 bp 1.0 61.9
ccsA-ndhD_p4 TGGATTCGGCAATTGTGGC CCTGATTTCGTTCTCTCGCT 52.6 50.0 59.1 57.7 410 bp 1.0 61.8
ccsA-ndhD_p5 GGCAATTGTGGCTTCTATAGGA TCCTGATTTCGTTCTCTCGC 45.5 50.0 58.2 57.7 404 bp 1.0 61.3