Hotspot

rpl32-trnL-UAG

SSC · 117251-118644 · 1394 bp

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Pi 0.00216
MarkerSeek score 53.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1394
variable_sites
3
pi
0.00216
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGCGGCAAAAGCTTTATCTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.7 57.9 1525-1527 bp 1.0 77.2
rpl32-trnL-UAG_p2 CGCGGCAAAAGCTTTATCTT AGCAGCGTGTCTACCAATTT 45.0 45.0 57.7 57.8 1518-1520 bp 1.0 76.7
rpl32-trnL-UAG_p3 CGCGGCAAAAGCTTTATCTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.7 57.8 1524-1526 bp 1.0 76.6
rpl32-trnL-UAG_p4 CGCGGCAAAAGCTTTATCTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.7 57.8 1523-1525 bp 1.0 76.6
rpl32-trnL-UAG_p5 CGCGGCAAAAGCTTTATCTT GAGCAGCGTGTCTACCAATT 45.0 50.0 57.7 58.3 1519-1521 bp 1.0 76.0