Hotspot

rpl20-rps12

LSC · 72354-73102 · 749 bp

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Pi 0.00134
MarkerSeek score 59.50
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
749
variable_sites
1
pi
0.00134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CTTGAATGAGCCCCTCGAAA AAATGTCACGAAATCCCCCG 50.0 50.0 57.9 58.5 905-906 bp 1.0 74.9
rpl20-rps12_p2 TTGAATGAGCCCCTCGAAAG AAATGTCACGAAATCCCCCG 50.0 50.0 57.9 58.5 904-905 bp 1.0 74.9
rpl20-rps12_p3 CTTGAATGAGCCCCTCGAAA CAGCGTCGAGGAACATGTAC 50.0 55.0 57.9 58.7 868-869 bp 1.0 73.3
rpl20-rps12_p4 TTGAATGAGCCCCTCGAAAG CAGCGTCGAGGAACATGTAC 50.0 55.0 57.9 58.7 867-868 bp 1.0 73.3
rpl20-rps12_p5 TTTGTTCTACGTCTCCGAGC AAATGTCACGAAATCCCCCG 50.0 50.0 57.7 58.5 863-864 bp 1.0 73.0