Hotspot

ycf4-cemA

LSC · 63393-64302 · 910 bp

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Pi 0.0011
MarkerSeek score 59.40
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
910
variable_sites
1
pi
0.0011
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 CATTCCCTTGACCCGTACTG AGATCCACCAGGGCAAAAAG 55.0 50.0 58.0 58.1 1083 bp 1.0 79.3
ycf4-cemA_p2 CATTCCCTTGACCCGTACTG GAGATCCACCAGGGCAAAAA 55.0 50.0 58.0 58.1 1084 bp 1.0 79.3
ycf4-cemA_p3 CATTCCCTTGACCCGTACTG GATCCACCAGGGCAAAAAGA 55.0 50.0 58.0 58.1 1082 bp 1.0 79.3
ycf4-cemA_p4 ATTCCCTTGACCCGTACTGA AGATCCACCAGGGCAAAAAG 50.0 50.0 58.1 58.1 1082 bp 1.0 79.1
ycf4-cemA_p5 ATTCCCTTGACCCGTACTGA GATCCACCAGGGCAAAAAGA 50.0 50.0 58.1 58.1 1081 bp 1.0 79.0