Hotspot

accD-psaI

LSC · 61700-62289 · 590 bp

Back to Aiouea

Pi 0.00171
MarkerSeek score 60.30
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
590
variable_sites
1
pi
0.00171
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AGCTACACGGTTTCTTTCCC GCCATTGCAATTGCTGGAAA 50.0 45.0 57.8 58.2 730-735 bp 1.0 77.7
accD-psaI_p2 GCTACACGGTTTCTTTCCCT GCCATTGCAATTGCTGGAAA 50.0 45.0 57.8 58.2 729-734 bp 1.0 77.7
accD-psaI_p3 AGCTACACGGTTTCTTTCCC AGAAGCCATTGCAATTGCTG 50.0 45.0 57.8 57.6 734-739 bp 1.0 76.2
accD-psaI_p4 GCTACACGGTTTCTTTCCCT AGAAGCCATTGCAATTGCTG 50.0 45.0 57.8 57.6 733-738 bp 1.0 76.2
accD-psaI_p5 GCTACACGGTTTCTTTCCCT TTGCTGGAAACACTAAGCCC 50.0 50.0 57.8 58.4 719-724 bp 1.0 76.2