Hotspot

rps4-trnT-UGU

LSC · 48259-48597 · 339 bp

Back to Aiouea

Pi 0.0059
MarkerSeek score 56.70
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
339
variable_sites
2
pi
0.0059
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 TCCCGAAACGTGATTGGTTT CTCAGAGGTTAGAGCATCGC 45.0 55.0 58.0 57.9 520 bp 1.0 79.0
rps4-trnT-UGU_p2 TTCCCGAAACGTGATTGGTT CTCAGAGGTTAGAGCATCGC 45.0 55.0 58.0 57.9 521 bp 1.0 79.0
rps4-trnT-UGU_p3 TTTCCCGAAACGTGATTGGT CTCAGAGGTTAGAGCATCGC 45.0 55.0 58.0 57.9 522 bp 1.0 79.0
rps4-trnT-UGU_p4 TCCCGAAACGTGATTGGTTT ATGGTCATCGGTTCGATTCC 45.0 50.0 58.0 57.8 488 bp 1.0 78.2
rps4-trnT-UGU_p5 TTCCCGAAACGTGATTGGTT ATGGTCATCGGTTCGATTCC 45.0 50.0 58.0 57.8 489 bp 1.0 78.2