Hotspot

matK-trnK-UUU

LSC · 3626-4345 · 720 bp

Back to Aiouea

Pi 0.00139
MarkerSeek score 59.50
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
720
variable_sites
1
pi
0.00139
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TTGTTGCCGAAATCCATCCA GTCGGGCAATCGAATCTCTT 45.0 50.0 58.1 58.1 869 bp 1.0 79.0
matK-trnK-UUU_p2 TGTTGCCGAAATCCATCCAT GTCGGGCAATCGAATCTCTT 45.0 50.0 57.9 58.1 868 bp 1.0 78.5
matK-trnK-UUU_p3 TTGTTGCCGAAATCCATCCA AACGGTAGAGTACTCGGCTT 45.0 50.0 58.1 58.2 792 bp 1.0 78.1
matK-trnK-UUU_p4 TTGTTGCCGAAATCCATCCA ACGGTAGAGTACTCGGCTTT 45.0 50.0 58.1 58.2 791 bp 1.0 78.1
matK-trnK-UUU_p5 TTGTTGCCGAAATCCATCCA ATGGGTTGCTAACTCAACGG 45.0 50.0 58.1 58.2 807 bp 1.0 78.0