primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score matK-trnK-UUU_p1 matK-trnK-UUU 1 TTGTTGCCGAAATCCATCCA GTCGGGCAATCGAATCTCTT 20 20 45.0 50.0 58.1 58.1 0.0 0.0 0.142169 3626 4345 869-869 1.000000 79.0 matK-trnK-UUU_p2 matK-trnK-UUU 2 TGTTGCCGAAATCCATCCAT GTCGGGCAATCGAATCTCTT 20 20 45.0 50.0 57.9 58.1 0.0 0.0 0.201031 3626 4345 868-868 1.000000 78.5 matK-trnK-UUU_p3 matK-trnK-UUU 3 TTGTTGCCGAAATCCATCCA AACGGTAGAGTACTCGGCTT 20 20 45.0 50.0 58.1 58.2 0.0 17.1 0.251914 3626 4345 792-792 1.000000 78.1 matK-trnK-UUU_p4 matK-trnK-UUU 4 TTGTTGCCGAAATCCATCCA ACGGTAGAGTACTCGGCTTT 20 20 45.0 50.0 58.1 58.2 0.0 17.1 0.251914 3626 4345 791-791 1.000000 78.1 matK-trnK-UUU_p5 matK-trnK-UUU 5 TTGTTGCCGAAATCCATCCA ATGGGTTGCTAACTCAACGG 20 20 45.0 50.0 58.1 58.2 0.0 5.8 0.262278 3626 4345 807-807 1.000000 78.0 trnG-UCC_p1 trnG-UCC 1 AACATGTCAGCACCCCAATT TCCGATTTCTTTCACGTTCTGA 20 22 45.0 40.9 58.0 58.0 0.0 0.0 2.029001 10319 11137 1037-1038 1.000000 63.9 trnG-UCC_p2 trnG-UCC 2 ACATGTCAGCACCCCAATTT TCCGATTTCTTTCACGTTCTGA 20 22 45.0 40.9 58.0 58.0 0.0 0.0 2.029001 10319 11137 1036-1037 1.000000 63.9 trnG-UCC_p3 trnG-UCC 3 AACATGTCAGCACCCCAATT CCGATTTCTTTCACGTTCTGAC 20 22 45.0 45.5 58.0 57.9 0.0 0.0 2.067912 10319 11137 1036-1037 1.000000 63.6 trnG-UCC_p4 trnG-UCC 4 ACATGTCAGCACCCCAATTT CCGATTTCTTTCACGTTCTGAC 20 22 45.0 45.5 58.0 57.9 0.0 0.0 2.067912 10319 11137 1035-1036 1.000000 63.6 trnG-UCC_p5 trnG-UCC 5 TGTCAGCACCCCAATTTTCA TCCGATTTCTTTCACGTTCTGA 20 22 45.0 40.9 57.9 58.0 0.0 0.0 2.094161 10319 11137 1033-1034 1.000000 63.4 atpF_p1 atpF 1 ACTAATTTCGTCGGCTCGAA GGGGGCGAAGTGATACAAAA 20 20 45.0 50.0 57.4 58.2 9.6 0.0 0.813159 13036 14317 1444-1444 1.000000 73.6 atpF_p2 atpF 2 ACTAATTTCGTCGGCTCGAA CAATCCAAAACAAAGGGGGC 20 20 45.0 50.0 57.4 57.8 9.6 0.0 0.825617 13036 14317 1458-1458 1.000000 73.5 atpF_p3 atpF 3 AATTTCGTCGGCTCGAATGG GGGGGCGAAGTGATACAAAA 20 20 50.0 50.0 59.0 58.2 13.2 0.0 1.164188 13036 14317 1441-1441 1.000000 70.8 atpF_p4 atpF 4 AATTTCGTCGGCTCGAATGG CAATCCAAAACAAAGGGGGC 20 20 50.0 50.0 59.0 57.8 13.2 0.0 1.176646 13036 14317 1455-1455 1.000000 70.7 atpF_p5 atpF 5 ACTAATTTCGTCGGCTCGAA CAAAGGGGGCGAAGTGATAC 20 20 45.0 55.0 57.4 58.6 9.6 0.0 1.262612 13036 14317 1448-1448 1.000000 70.0 rpoC2_p1 rpoC2 1 GGAACGGGGCATTAGTTCTT TTTCTGACCTCTCCGTGACT 20 20 50.0 50.0 57.9 58.0 11.4 0.0 0.127479 19989 20108 248-248 1.000000 79.3 rpoC2_p2 rpoC2 2 GGAACGGGGCATTAGTTCTT TTCGCTCCACAAGGATCAAG 20 20 50.0 50.0 57.9 57.9 11.4 0.0 0.223418 19989 20108 304-304 1.000000 78.5 rpoC2_p3 rpoC2 3 GGAACGGGGCATTAGTTCTT CGCTCCACAAGGATCAAGAT 20 20 50.0 50.0 57.9 57.7 11.4 0.0 0.446633 19989 20108 302-302 1.000000 76.7 rpoC2_p4 rpoC2 4 GGAACGGGGCATTAGTTCTT CCTTTTTCGCTCCACAAGGA 20 20 50.0 50.0 57.9 58.4 11.4 0.0 0.519673 19989 20108 309-309 1.000000 76.1 rpoC2_p5 rpoC2 5 GGAACGGGGCATTAGTTCTT TAGTGCCTTTTTCGCTCCAC 20 20 50.0 50.0 57.9 58.5 11.4 0.0 0.604869 19989 20108 314-314 1.000000 75.4 petN-psbM_p1 petN-psbM 1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCGTTGTTCATTCT 20 21 50.0 42.9 58.2 58.2 0.0 0.0 1.385036 29968 31030 1225-1225 1.000000 69.1 petN-psbM_p2 petN-psbM 2 TAGTAAGTCTCGCTTGGGCT TGCTACTGCGTTGTTCATTC 20 20 50.0 45.0 58.2 56.7 0.0 0.0 1.402106 29968 31030 1225-1225 1.000000 68.9 petN-psbM_p3 petN-psbM 3 GGGACACGATTCACATGGAT TGCTACTGCGTTGTTCATTCT 20 21 50.0 42.9 57.7 58.2 0.0 0.0 1.565751 29968 31030 1246-1246 1.000000 67.6 petN-psbM_p4 petN-psbM 4 GGGACACGATTCACATGGAT TGCTACTGCGTTGTTCATTC 20 20 50.0 45.0 57.7 56.7 0.0 0.0 1.582821 29968 31030 1246-1246 1.000000 67.5 petN-psbM_p5 petN-psbM 5 ATGGGGAAGAAGTGGACTCT TGCTACTGCGTTGTTCATTCT 20 21 50.0 42.9 57.6 58.2 0.0 0.0 1.584550 29968 31030 1164-1164 1.000000 67.5 rps4-trnT-UGU_p1 rps4-trnT-UGU 1 TCCCGAAACGTGATTGGTTT CTCAGAGGTTAGAGCATCGC 20 20 45.0 55.0 58.0 57.9 7.0 0.0 0.162648 48259 48597 520-520 1.000000 79.0 rps4-trnT-UGU_p2 rps4-trnT-UGU 2 TTCCCGAAACGTGATTGGTT CTCAGAGGTTAGAGCATCGC 20 20 45.0 55.0 58.0 57.9 7.0 0.0 0.162648 48259 48597 521-521 1.000000 79.0 rps4-trnT-UGU_p3 rps4-trnT-UGU 3 TTTCCCGAAACGTGATTGGT CTCAGAGGTTAGAGCATCGC 20 20 45.0 55.0 58.0 57.9 7.0 0.0 0.162648 48259 48597 522-522 1.000000 79.0 rps4-trnT-UGU_p4 rps4-trnT-UGU 4 TCCCGAAACGTGATTGGTTT ATGGTCATCGGTTCGATTCC 20 20 45.0 50.0 58.0 57.8 7.0 0.0 0.270402 48259 48597 488-488 1.000000 78.2 rps4-trnT-UGU_p5 rps4-trnT-UGU 5 TTCCCGAAACGTGATTGGTT ATGGTCATCGGTTCGATTCC 20 20 45.0 50.0 58.0 57.8 7.0 0.0 0.270402 48259 48597 489-489 1.000000 78.2 ndhK_p1 ndhK 1 ATGAACGACCCTGCATCTTC GGGGGATGCTTGGTTACAAT 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.189017 51327 52184 1063-1063 1.000000 78.6 ndhK_p2 ndhK 2 GATGAACGACCCTGCATCTT GGGGGATGCTTGGTTACAAT 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.189017 51327 52184 1064-1064 1.000000 78.6 ndhK_p3 ndhK 3 GAACGACCCTGCATCTTCTT GGGGGATGCTTGGTTACAAT 20 20 50.0 50.0 57.9 57.8 0.0 0.0 0.259784 51327 52184 1061-1061 1.000000 78.0 ndhK_p4 ndhK 4 ATGAACGACCCTGCATCTTC GATGGGGGATGCTTGGTTAC 20 20 50.0 55.0 58.0 58.3 0.0 0.0 0.337428 51327 52184 1066-1066 1.000000 77.4 ndhK_p5 ndhK 5 GATGAACGACCCTGCATCTT GATGGGGGATGCTTGGTTAC 20 20 50.0 55.0 58.0 58.3 0.0 0.0 0.337428 51327 52184 1067-1067 1.000000 77.4 accD-psaI_p1 accD-psaI 1 AGCTACACGGTTTCTTTCCC GCCATTGCAATTGCTGGAAA 20 20 50.0 45.0 57.8 58.2 0.0 32.1 0.369473 61700 62289 730-735 1.000000 77.7 accD-psaI_p2 accD-psaI 2 GCTACACGGTTTCTTTCCCT GCCATTGCAATTGCTGGAAA 20 20 50.0 45.0 57.8 58.2 0.0 32.1 0.369473 61700 62289 729-734 1.000000 77.7 accD-psaI_p3 accD-psaI 3 AGCTACACGGTTTCTTTCCC AGAAGCCATTGCAATTGCTG 20 20 50.0 45.0 57.8 57.6 0.0 31.8 0.560516 61700 62289 734-739 1.000000 76.2 accD-psaI_p4 accD-psaI 4 GCTACACGGTTTCTTTCCCT AGAAGCCATTGCAATTGCTG 20 20 50.0 45.0 57.8 57.6 0.0 31.8 0.560516 61700 62289 733-738 1.000000 76.2 accD-psaI_p5 accD-psaI 5 GCTACACGGTTTCTTTCCCT TTGCTGGAAACACTAAGCCC 20 20 50.0 50.0 57.8 58.4 0.0 0.0 0.564273 61700 62289 719-724 1.000000 76.2 ycf4-cemA_p1 ycf4-cemA 1 CATTCCCTTGACCCGTACTG AGATCCACCAGGGCAAAAAG 20 20 55.0 50.0 58.0 58.1 0.0 0.0 0.095307 63393 64302 1083-1083 1.000000 79.3 ycf4-cemA_p2 ycf4-cemA 2 CATTCCCTTGACCCGTACTG GAGATCCACCAGGGCAAAAA 20 20 55.0 50.0 58.0 58.1 0.0 0.0 0.100820 63393 64302 1084-1084 1.000000 79.3 ycf4-cemA_p3 ycf4-cemA 3 CATTCCCTTGACCCGTACTG GATCCACCAGGGCAAAAAGA 20 20 55.0 50.0 58.0 58.1 0.0 0.0 0.100820 63393 64302 1082-1082 1.000000 79.3 ycf4-cemA_p4 ycf4-cemA 4 ATTCCCTTGACCCGTACTGA AGATCCACCAGGGCAAAAAG 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.124147 63393 64302 1082-1082 1.000000 79.1 ycf4-cemA_p5 ycf4-cemA 5 ATTCCCTTGACCCGTACTGA GATCCACCAGGGCAAAAAGA 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.129660 63393 64302 1081-1081 1.000000 79.0 cemA_p1 cemA 1 ATAGAGTCGGCGAATGAAGC CCTGGGCGGGTAGAAGTATA 20 20 50.0 55.0 57.9 58.0 16.9 0.0 0.146905 64303 64992 842-842 1.000000 78.9 cemA_p2 cemA 2 GGATCAGATAGAGTCGGCGA CCTGGGCGGGTAGAAGTATA 20 20 55.0 55.0 58.5 58.0 5.0 0.0 0.480522 64303 64992 849-849 1.000000 76.3 cemA_p3 cemA 3 TCGGATCAGATAGAGTCGGC CCTGGGCGGGTAGAAGTATA 20 20 55.0 55.0 58.5 58.0 0.0 0.0 0.480522 64303 64992 851-851 1.000000 76.2 cemA_p4 cemA 4 CAGATAGAGTCGGCGAATGA CCTGGGCGGGTAGAAGTATA 20 20 50.0 55.0 56.9 58.0 16.9 0.0 1.072758 64303 64992 845-845 1.000000 71.5 cemA_p5 cemA 5 TCAGATAGAGTCGGCGAATG CCTGGGCGGGTAGAAGTATA 20 20 50.0 55.0 56.9 58.0 16.9 0.0 1.072758 64303 64992 846-846 1.000000 71.5 petA-psbJ_p1 petA-psbJ 1 ACGTGTCCAAGGTTTGTTGT GGCTCATATTCCGGATTGGG 20 20 45.0 55.0 58.2 58.2 0.0 4.1 0.344636 66178 67319 1287-1288 1.000000 78.1 petA-psbJ_p2 petA-psbJ 2 ACGTGTCCAAGGTTTGTTGT CGGCTCATATTCCGGATTGG 20 20 45.0 55.0 58.2 58.6 0.0 4.1 0.728252 66178 67319 1288-1289 1.000000 75.0 petA-psbJ_p3 petA-psbJ 3 ACGTGTCCAAGGTTTGTTGT GCTCATATTCCGGATTGGGA 20 20 45.0 50.0 58.2 57.1 0.0 4.1 1.030186 66178 67319 1286-1287 1.000000 72.6 petA-psbJ_p4 petA-psbJ 4 ACGTGTCCAAGGTTTGTTGT ATTCCGGATTGGGATCATCC 20 20 45.0 50.0 58.2 57.1 0.0 4.1 1.058172 66178 67319 1280-1281 1.000000 72.4 petA-psbJ_p5 petA-psbJ 5 CGTGTCCAAGGTTTGTTGTT GGCTCATATTCCGGATTGGG 20 20 45.0 55.0 57.1 58.2 0.0 4.1 1.105127 66178 67319 1286-1287 1.000000 72.0 rpl20-rps12_p1 rpl20-rps12 1 CTTGAATGAGCCCCTCGAAA AAATGTCACGAAATCCCCCG 20 20 50.0 50.0 57.9 58.5 0.0 0.0 0.654410 72354 73102 905-906 1.000000 74.9 rpl20-rps12_p2 rpl20-rps12 2 TTGAATGAGCCCCTCGAAAG AAATGTCACGAAATCCCCCG 20 20 50.0 50.0 57.9 58.5 0.0 0.0 0.654410 72354 73102 904-905 1.000000 74.9 rpl20-rps12_p3 rpl20-rps12 3 CTTGAATGAGCCCCTCGAAA CAGCGTCGAGGAACATGTAC 20 20 50.0 55.0 57.9 58.7 0.0 9.8 0.844694 72354 73102 868-869 1.000000 73.3 rpl20-rps12_p4 rpl20-rps12 4 TTGAATGAGCCCCTCGAAAG CAGCGTCGAGGAACATGTAC 20 20 50.0 55.0 57.9 58.7 0.0 9.8 0.844694 72354 73102 867-868 1.000000 73.3 rpl20-rps12_p5 rpl20-rps12 5 TTTGTTCTACGTCTCCGAGC AAATGTCACGAAATCCCCCG 20 20 50.0 50.0 57.7 58.5 0.0 0.0 0.883460 72354 73102 863-864 1.000000 73.0 ycf2_p1 ycf2 1 CAGCCCTTTGTCTCTGTGTT TATTCCATCGATCCCCACGA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.031065 96584 96703 598-598 1.000000 82.6 ycf2_p2 ycf2 2 GTTCTTGCAGAGTGGAACCA TATTCCATCGATCCCCACGA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.036618 96584 96703 722-722 1.000000 82.0 ycf2_p3 ycf2 3 CAGCCCTTTGTCTCTGTGTT AAATGGTAGACACGCGAGAC 20 20 50.0 50.0 58.0 58.0 0.0 15.9 0.034958 96584 96703 762-762 1.000000 81.9 ycf2_p4 ycf2 4 CAGCCCTTTGTCTCTGTGTT TGAAATGGTAGACACGCGAG 20 20 50.0 50.0 58.0 58.0 0.0 15.9 0.036329 96584 96703 764-764 1.000000 81.9 ycf2_p5 ycf2 5 GTTCTTGCAGAGTGGAACCA AAATGGTAGACACGCGAGAC 20 20 50.0 50.0 58.0 58.0 0.0 15.9 0.040512 96584 96703 886-886 1.000000 81.6 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 CGCGGCAAAAGCTTTATCTT TCCTAAGAGCAGCGTGTCTA 20 20 45.0 50.0 57.7 57.9 13.1 0.0 0.386849 117251 118644 1525-1527 1.000000 77.2 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 CGCGGCAAAAGCTTTATCTT AGCAGCGTGTCTACCAATTT 20 20 45.0 45.0 57.7 57.8 13.1 0.0 0.447576 117251 118644 1518-1520 1.000000 76.7 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 CGCGGCAAAAGCTTTATCTT CCTAAGAGCAGCGTGTCTAC 20 20 45.0 55.0 57.7 57.8 13.1 0.0 0.458867 117251 118644 1524-1526 1.000000 76.6 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 CGCGGCAAAAGCTTTATCTT CTAAGAGCAGCGTGTCTACC 20 20 45.0 55.0 57.7 57.8 13.1 0.0 0.458867 117251 118644 1523-1525 1.000000 76.6 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 CGCGGCAAAAGCTTTATCTT GAGCAGCGTGTCTACCAATT 20 20 45.0 50.0 57.7 58.3 13.1 0.0 0.535719 117251 118644 1519-1521 1.000000 76.0 trnL-UAG-trnI-CAU_p1 trnL-UAG-trnI-CAU 1 TTATTTCGATGAAGCCGCCA TAGAATTTGGGTCACTCGCG 20 20 45.0 50.0 58.0 58.0 0.0 0.0 0.035841 118725 118767 251-251 1.000000 80.0 trnL-UAG-trnI-CAU_p2 trnL-UAG-trnI-CAU 2 TTATTTCGATGAAGCCGCCA ACTCGCGATGTACAATGACC 20 20 45.0 50.0 58.0 58.1 0.0 10.4 0.108688 118725 118767 238-238 1.000000 79.5 trnL-UAG-trnI-CAU_p3 trnL-UAG-trnI-CAU 3 TTATTTCGATGAAGCCGCCA GGTCACTCGCGATGTACAAT 20 20 45.0 50.0 58.0 58.1 0.0 10.4 0.108688 118725 118767 242-242 1.000000 79.5 trnL-UAG-trnI-CAU_p4 trnL-UAG-trnI-CAU 4 TAGACACGCTGCTCTTAGGA TAGAATTTGGGTCACTCGCG 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.128860 118725 118767 219-219 1.000000 79.3 trnL-UAG-trnI-CAU_p5 trnL-UAG-trnI-CAU 5 TTATTTCGATGAAGCCGCCA AATGACCCCAGTGAAGCATC 20 20 45.0 50.0 58.0 57.9 0.0 0.0 0.166047 118725 118767 225-225 1.000000 79.0 ccsA_p1 ccsA 1 CGCGAGTGACCCAAATTCTA TTCTCGATGGCTTCTCTTGC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.020318 118945 119895 1618-1618 1.000000 80.1 ccsA_p2 ccsA 2 TTATTTCGATGAAGCCGCCA TTCTCGATGGCTTCTCTTGC 20 20 45.0 50.0 58.0 58.0 0.0 0.0 0.046994 118945 119895 1849-1849 1.000000 79.9 ccsA_p3 ccsA 3 CGCGAGTGACCCAAATTCTA CTCGATGGCTTCTCTTGCAT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.053754 118945 119895 1616-1616 1.000000 79.9 ccsA_p4 ccsA 4 CGCGAGTGACCCAAATTCTA TTGCATTGCCAGGAATGAGT 20 20 50.0 45.0 58.0 58.1 0.0 15.1 0.077095 118945 119895 1602-1602 1.000000 79.7 ccsA_p5 ccsA 5 TTATTTCGATGAAGCCGCCA CTCGATGGCTTCTCTTGCAT 20 20 45.0 50.0 58.0 58.0 0.0 0.0 0.080430 118945 119895 1847-1847 1.000000 79.7 ccsA-ndhD_p1 ccsA-ndhD 1 GTGGATTCGGCAATTGTGG TCCTGATTTCGTTCTCTCGC 19 20 52.6 50.0 57.3 57.7 7.4 0.0 1.999282 119896 120117 412-412 1.000000 65.0 ccsA-ndhD_p2 ccsA-ndhD 2 GTGGATTCGGCAATTGTGG CCTGATTTCGTTCTCTCGCT 19 20 52.6 50.0 57.3 57.7 7.4 0.0 2.005526 119896 120117 411-411 1.000000 64.9 ccsA-ndhD_p3 ccsA-ndhD 3 TGGATTCGGCAATTGTGGC TCCTGATTTCGTTCTCTCGC 19 20 52.6 50.0 59.1 57.7 7.4 0.0 2.390128 119896 120117 411-411 1.000000 61.9 ccsA-ndhD_p4 ccsA-ndhD 4 TGGATTCGGCAATTGTGGC CCTGATTTCGTTCTCTCGCT 19 20 52.6 50.0 59.1 57.7 7.4 0.0 2.396373 119896 120117 410-410 1.000000 61.8 ccsA-ndhD_p5 ccsA-ndhD 5 GGCAATTGTGGCTTCTATAGGA TCCTGATTTCGTTCTCTCGC 22 20 45.5 50.0 58.2 57.7 7.4 0.0 2.459025 119896 120117 404-404 1.000000 61.3 trnL-CAA-ycf2_p1 trnL-CAA-ycf2 1 AAATGGTAGACACGCGAGAC CAGCCCTTTGTCTCTGTGTT 20 20 50.0 50.0 58.0 58.0 15.9 0.0 0.034958 149192 149530 762-762 1.000000 81.9 trnL-CAA-ycf2_p2 trnL-CAA-ycf2 2 TGAAATGGTAGACACGCGAG CAGCCCTTTGTCTCTGTGTT 20 20 50.0 50.0 58.0 58.0 15.9 0.0 0.036329 149192 149530 764-764 1.000000 81.9 trnL-CAA-ycf2_p3 trnL-CAA-ycf2 3 AAATGGTAGACACGCGAGAC GTTCTTGCAGAGTGGAACCA 20 20 50.0 50.0 58.0 58.0 15.9 0.0 0.040512 149192 149530 886-886 1.000000 81.6 trnL-CAA-ycf2_p4 trnL-CAA-ycf2 4 TGAAATGGTAGACACGCGAG GTTCTTGCAGAGTGGAACCA 20 20 50.0 50.0 58.0 58.0 15.9 0.0 0.041882 149192 149530 888-888 1.000000 81.6 trnL-CAA-ycf2_p5 trnL-CAA-ycf2 5 AAATGGTAGACACGCGAGAC TATTGGGCCAGGTCATTTCG 20 20 50.0 50.0 58.0 57.9 15.9 6.2 0.059165 149192 149530 960-960 1.000000 81.3