Hotspot

ycf1

SSC · 123188-128296 · 5109 bp

Back to Ainsliaea

Pi 0.000587

MarkerSeek score 52.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5109
variable_sites: 3
pi: 0.000587
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACTGTATTTCTATGCACACCA	GGACCAAAGTAATCAAAGATACCG	38.1	41.7	54.9	57.6	225 bp	1.0	40.4
ycf1_p2	TGTTCTCCTTGCAAAATACTGT	GGACCAAAGTAATCAAAGATACCG	36.4	41.7	56.2	57.6	242 bp	1.0	40.3
ycf1_p3	TGTTCTCCTTGCAAAATACTGT	CCTTGGTTCATAAAAGTAAGGACC	36.4	41.7	56.2	57.5	261 bp	1.0	40.3
ycf1_p4	CTTGTTCTCCTTGCAAAATACTGT	GGACCAAAGTAATCAAAGATACCG	37.5	41.7	57.9	57.6	244 bp	1.0	40.3
ycf1_p5	TTGTTCTCCTTGCAAAATACTGT	GGACCAAAGTAATCAAAGATACCG	34.8	41.7	56.9	57.6	243 bp	1.0	40.3