Hotspot

ndhD

SSC · 115108-116628 · 1521 bp

Back to Ainsliaea

Pi 0.000657

MarkerSeek score 58.30

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1521
variable_sites: 1
pi: 0.000657
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCATTAGCCAACCAACTCTT	CCAAAACCTGTGCCCAAAAA	40.0	45.0	54.5	57.6	1628 bp	1.0	49.1
ndhD_p2	TTCATTAGCCAACCAACTCT	CCAAAACCTGTGCCCAAAAA	40.0	45.0	54.5	57.6	1629 bp	1.0	49.1
ndhD_p3	TTCATTAGCCAACCAACTCTT	CCAAAACCTGTGCCCAAAAA	38.1	45.0	55.4	57.6	1629 bp	1.0	47.9
ndhD_p4	TTTCATTAGCCAACCAACTCT	CCAAAACCTGTGCCCAAAAA	38.1	45.0	55.4	57.6	1630 bp	1.0	47.9
ndhD_p5	TCATTAGCCAACCAACTCTT	CACGGGTTTTTCTGGTCCAA	40.0	50.0	54.5	58.6	1593 bp	1.0	47.6