Hotspot

cemA

LSC · 61137-61826 · 690 bp

Back to Ainsliaea

Pi 0.00145
MarkerSeek score 59.60
Primer available yes
Label cemA

Feature Metadata

label_name
cemA
feature_type
gene
region
LSC
length_bp
690
variable_sites
1
pi
0.00145
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA_p1 GAGTCGACGAATGAGATGGG GCTTTGCTTATGTACAACTGCA 55.0 40.9 57.9 57.8 797 bp 1.0 61.8
cemA_p2 GAGTCGACGAATGAGATGGG TGCTTTGCTTATGTACAACTGC 55.0 40.9 57.9 57.8 798 bp 1.0 61.8
cemA_p3 CGACGAATGAGATGGGTTCA GCTTTGCTTATGTACAACTGCA 50.0 40.9 57.7 57.8 793 bp 1.0 60.5
cemA_p4 CGACGAATGAGATGGGTTCA TGCTTTGCTTATGTACAACTGC 50.0 40.9 57.7 57.8 794 bp 1.0 60.5
cemA_p5 GTCGACGAATGAGATGGGTT GCTTTGCTTATGTACAACTGCA 50.0 40.9 57.7 57.8 795 bp 1.0 60.5