Hotspot

ycf56

SSC · 53972-54664 · 693 bp

Back to Ahnfeltia

Pi 0.468

MarkerSeek score 80.70

Primer available yes

Label ycf56

Feature Metadata

label_name: ycf56
feature_type: gene
region: SSC
length_bp: 693
variable_sites: 275
pi: 0.468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf56_p1	GCCCAGGTTCAACCCATAAT	CATTGCTTACGGGAAGGTCA	50.0	50.0	57.8	57.9	1070 bp	0.5	57.9
ycf56_p2	GCCCAGGTTCAACCCATAAT	TTGCTTACGGGAAGGTCAAC	50.0	50.0	57.8	58.1	1068 bp	0.5	57.8
ycf56_p3	AGCAAGCACAATCTGACCTC	CATTGCTTACGGGAAGGTCA	50.0	50.0	58.2	57.9	1447 bp	0.5	57.7
ycf56_p4	AGCAAGCACAATCTGACCTC	TTGCTTACGGGAAGGTCAAC	50.0	50.0	58.2	58.1	1445 bp	0.5	57.6
ycf56_p5	CAAGCACAATCTGACCTCCA	CATTGCTTACGGGAAGGTCA	50.0	50.0	57.8	57.9	1445 bp	0.5	57.6