Hotspot

ycf55

SSC · 56888-57853 · 966 bp

Back to Ahnfeltia

Pi 0.465

MarkerSeek score 82.30

Primer available yes

Label ycf55

Feature Metadata

label_name: ycf55
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 404
pi: 0.465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf55_p1	GCTTAGGTGCTGCTTTTGTC	GTCCAGCCGATTTTATCCCA	50.0	50.0	57.7	57.7	2379 bp	0.5	54.6
ycf55_p2	GCTTAGGTGCTGCTTTTGTC	TGTCCAGCCGATTTTATCCC	50.0	50.0	57.7	57.7	2380 bp	0.5	54.6
ycf55_p3	AGGTGCTGCTTTTGTCTCTG	GTCCAGCCGATTTTATCCCA	50.0	50.0	58.4	57.7	2375 bp	0.5	54.1
ycf55_p4	AGGTGCTGCTTTTGTCTCTG	TGTCCAGCCGATTTTATCCC	50.0	50.0	58.4	57.7	2376 bp	0.5	54.1
ycf55_p5	TCAATCTGGCTTAGGTGCTG	GTCCAGCCGATTTTATCCCA	50.0	50.0	57.6	57.7	2387 bp	0.5	54.0