Hotspot

ycf46

SSC · 67153-68637 · 1485 bp

Back to Ahnfeltia

Pi 0.495

MarkerSeek score 81.70

Primer available yes

Label ycf46

Feature Metadata

label_name: ycf46
feature_type: gene
region: SSC
length_bp: 1485
variable_sites: 648
pi: 0.495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf46_p1	AAGGTCGGAAAAGAATCGGC	GAAGCCCACCTCAAGATGAG	50.0	55.0	58.5	58.0	1952 bp	0.5	55.3
ycf46_p2	GGTCGGAAAAGAATCGGCTT	GAAGCCCACCTCAAGATGAG	50.0	55.0	58.5	58.0	1950 bp	0.5	55.3
ycf46_p3	AAGGTCGGAAAAGAATCGGC	TCTCCCCCAAGAGTTCACAT	50.0	50.0	58.5	58.0	2256 bp	0.5	55.2
ycf46_p4	GGTCGGAAAAGAATCGGCTT	ATCTCCCCCAAGAGTTCACA	50.0	50.0	58.5	58.0	2255 bp	0.5	55.2
ycf46_p5	GGTCGGAAAAGAATCGGCTT	TCTCCCCCAAGAGTTCACAT	50.0	50.0	58.5	58.0	2254 bp	0.5	55.2