Hotspot

thiG

SSC · 43544-44392 · 849 bp

Back to Ahnfeltia

Pi 0.499

MarkerSeek score 79.80

Primer available yes

Label thiG

Feature Metadata

label_name: thiG
feature_type: gene
region: SSC
length_bp: 849
variable_sites: 346
pi: 0.499
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
thiG_p1	TCGAAACGTAGCACCCAAAA	CTTCCGGGTCCAGGAGATAT	45.0	55.0	58.0	58.0	1692 bp	0.5	59.5
thiG_p2	TCGAAACGTAGCACCCAAAA	CTGGCTGGCCTAACGAATAG	45.0	55.0	58.0	58.1	2176 bp	0.5	58.6
thiG_p3	TCGAAACGTAGCACCCAAAA	GGCTGGCCTAACGAATAGAG	45.0	55.0	58.0	57.8	2174 bp	0.5	58.4
thiG_p4	TCGAAACGTAGCACCCAAAA	ATTGAACTGGCTGGCCTAAC	45.0	50.0	58.0	58.2	2182 bp	0.5	58.3
thiG_p5	TCGAAACGTAGCACCCAAAA	ACCTGAATCAGTTGGGCTTC	45.0	50.0	58.0	57.8	1708 bp	0.5	58.0