Hotspot

rbcR

SSC · 55415-56365 · 951 bp

Back to Ahnfeltia

Pi 0.456

MarkerSeek score 81.20

Primer available yes

Label rbcR

Feature Metadata

label_name: rbcR
feature_type: gene
region: SSC
length_bp: 951
variable_sites: 376
pi: 0.456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcR_p1	CGAAGTTGCTGTAAAGCTGC	AAGACTTTGCTCTGAAGGCC	50.0	50.0	58.0	58.1	1831 bp	0.5	58.9
rbcR_p2	TGACCTTCCCGTAAGCAATG	AAGACTTTGCTCTGAAGGCC	50.0	50.0	57.9	58.1	2122 bp	0.5	58.4
rbcR_p3	GTTGACCTTCCCGTAAGCAA	AAGACTTTGCTCTGAAGGCC	50.0	50.0	58.1	58.1	2124 bp	0.5	58.3
rbcR_p4	AGCTAGTTGACCTTCCCGTA	AAGACTTTGCTCTGAAGGCC	50.0	50.0	57.8	58.1	2129 bp	0.5	57.4
rbcR_p5	CGAAGTTGCTGTAAAGCTGC	ACTTTGCTCTGAAGGCCAAT	50.0	45.0	58.0	57.7	1828 bp	0.5	57.3