Hotspot

ccs1

SSC · 44980-46287 · 1308 bp

Back to Ahnfeltia

Pi 0.463

MarkerSeek score 83.20

Primer available yes

Label ccs1

Feature Metadata

label_name: ccs1
feature_type: gene
region: SSC
length_bp: 1308
variable_sites: 565
pi: 0.463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccs1_p1	ATATCTCCTGGACCCGGAAG	CATACGGATCGATGGACTGC	55.0	55.0	58.0	58.3	2198 bp	0.5	57.5
ccs1_p2	ACTCAATACTGCAGTAGCGC	CATACGGATCGATGGACTGC	50.0	55.0	58.1	58.3	2537 bp	0.5	57.0
ccs1_p3	CTCAATACTGCAGTAGCGCA	CATACGGATCGATGGACTGC	50.0	55.0	58.1	58.3	2536 bp	0.5	57.0
ccs1_p4	ATATCTCCTGGACCCGGAAG	CGGATCGATGGACTGCAAAT	55.0	50.0	58.0	58.4	2194 bp	0.5	56.6
ccs1_p5	ACTCAATACTGCAGTAGCGC	CGGATCGATGGACTGCAAAT	50.0	50.0	58.1	58.4	2533 bp	0.5	56.1