Hotspot

ccdA

SSC · 46406-47131 · 726 bp

Back to Ahnfeltia

Pi 0.476

MarkerSeek score 82.40

Primer available yes

Label ccdA

Feature Metadata

label_name: ccdA
feature_type: gene
region: SSC
length_bp: 726
variable_sites: 313
pi: 0.476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccdA_p1	TGAAACAACTCACCACTGGG	GCTTGCGAAAAATGGTGGAA	50.0	45.0	57.9	57.9	2181 bp	0.5	58.4
ccdA_p2	ATTTCCAGAATTGTCCGCGA	GCTTGCGAAAAATGGTGGAA	45.0	45.0	57.9	57.9	1763 bp	0.5	58.0
ccdA_p3	TGAAACAACTCACCACTGGG	GAGCTTGCGAAAAATGGTGG	50.0	50.0	57.9	58.3	2183 bp	0.5	57.2
ccdA_p4	ATTTCCAGAATTGTCCGCGA	GAGCTTGCGAAAAATGGTGG	45.0	50.0	57.9	58.3	1765 bp	0.5	56.8
ccdA_p5	TGAAACAACTCACCACTGGG	ATATTTCTTCGGGTGCAGCA	50.0	45.0	57.9	57.6	2147 bp	0.5	56.2